if you are running the netfilter implementation, I think the iptables mangle is handled automatically.(I verified this by changing my Jool to netfilter and removed these lines below:
ip6tables -t mangle -A PREROUTING --destination 64:ff9b::/96 -j JOOL --instance default iptables -t mangle -A PREROUTING --destination 172.21.8.202 -p tcp --dport 1:65535 -j JOOL --instance default iptables -t mangle -A PREROUTING --destination 172.21.8.202 -p udp --dport 1:65535 -j JOOL --instance default iptables -t mangle -A PREROUTING --destination 172.21.8.202 -p icmp -j JOOL --instance default